Key result
Increasing cell densities reduced FMDV viral titer and increased yield variability in ACF and CDM media, an effect mitigated by 100% media exchange before infection or bioreactor use.
Population
Cells grown at increasing cell densities for Foot-and-mouth disease virus (FMDV) propagation
Comparison
100% media exchange or addition of 30% fresh… vs No media exchange or different media types
Design
Preclinical
Authors
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May inform high-density FMDV vaccine manufacturing; hypothesis-generating and leaves open clinical-scale validation.
In FMDV vaccine production, cell density effects that reduce viral titer can be mitigated by complete media exchange prior to infection.
Dill et al. (2019) studied Foot-and-mouth disease virus (FMDV) propagation. Animal-component-free (ACF) or chemically defined media (CDM) at increasing cell densities vs. BHK300G media / lower cell densities was evaluated on Viral titer and yield variability. Increasing cell densities reduced FMDV viral titer and increased yield variability in ACF and CDM media, an effect mitigated by 100% media exchange before infection or bioreactor use.
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