September 1, 1967Journal of HygieneOpen Access

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

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Population

BHK21 Clone 13 cells in deep suspension cultures

Design

Preclinical

Authors

PCP. B. CapstickThe Pirbright Institute AGA.J.M. GarlandRoyal Cornwall Hospital WCW. G. ChapmanHuntsville Hospital

Discussion

Member takes

Implication

May improve FMDV yields in BHK21 cells; hypothesis-generating for bioprocess scale-up without clinical validation.

Structured PICO

Population

BHK21 Clone 13 cells in deep suspension cultures

Intervention

Optimization of culture conditions including temperature (34.25-35°C), pH (7.2), cell concentration (1x10^6 to 9x10^6 cells/ml), input virus/cell ratio (1:1 to 1:320), and addition of 5% serum

Outcome

Foot-and-mouth disease (FMD) virus production/yield

Optimizing culture temperature, pH, cell concentration, and serum addition maximizes foot-and-mouth disease virus production in BHK21 Clone 13 cells.

Cite This Study

Capstick et al. (1967) studied this question.

synapsesocial.com/papers/6a880d668c7e900eb714bb4c https://doi.org/10.1017/s0022172400045794

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Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Factors affecting the production of foot-and-mouth disease virus in deep suspension cultures of BHK21 Clone 13 cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider