Key result
Suspension-adapted BHK-21 cells in serum-free medium reached a viable cell density of 7.65 × 10^6 cells/mL on day 3 and improved FMD virus productivity.
Why the study?
BHK-21 cells are anchorage-dependent, whereas suspension cultures would enable rapid growth in bioreactors, large-scale virus propagation, and cost-effective vaccine production with serum-free medium.
Population
BHK-21 cell line
Comparison
Suspension-adapted BHK-21 cells vs adherent BHK-21 cells
Authors
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Suspension-adapted BHK-21 cells reach high density for FMD vaccines; leaves open serum-free bioreactor scalability.
Adapting BHK-21 cells to suspension culture in serum-free medium enables large-scale, cost-effective production of foot-and-mouth disease vaccine antigen.
Park et al. (2021) studied Foot-and-mouth disease. Suspension-adapted BHK-21 cells vs. Adherent BHK-21 cells was evaluated on Viable cell density and FMD virus production yields. Suspension-adapted BHK-21 cells in serum-free medium reached a viable cell density of 7.65 × 10^6 cells/mL on day 3 and improved FMD virus productivity.
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