Key result
Production of foot-and-mouth disease virus in BHK-21 cells adapted to serum-free chemically defined medium yielded peak harvest infectivities of approximately 10^8.5 PFU/ml.
Population
Baby hamster kidney suspension cells adapted to and continually passed in a glutamine-free autoclavable…
Design
Preclinical
Authors
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Serum-free FMDV production in adapted BHK cells reaches high titers; leaves open scalable GMP validation for vaccine manufacturing.
Foot-and-mouth disease virus can be successfully propagated in BHK cells using a serum-free chemically defined medium, producing high viral titers suitable for vaccine production.
Tomei et al. (1975) studied Foot-and-mouth disease virus production. FMD virus production in BHK-21 cells in serum-free chemically defined medium was evaluated on Peak harvest infectivities and complement-fixing antigen produced. Production of foot-and-mouth disease virus in BHK-21 cells adapted to serum-free chemically defined medium yielded peak harvest infectivities of approximately 10^8.5 PFU/ml.
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