Key result
BHK-21 monolayer cell culture in roller bottles yielded higher biological titers (TCID50 up to 10^-9.83/ml) for FMD virus serotypes O, A, and Asia-1 compared to suspension cells.
Why the study?
This study was designed to ensure the production of quality foot-and-mouth disease vaccine by determining the biological titer of virus serotypes in cell lines.
Population
BHK-21 Monolayer and BHK-21 Clone 13 Suspension cells infected with FMDV serotypes O, A, and Asia-1
Comparison
BHK-21 monolayer cell culture vs BHK-21 Clone 13 suspension cell culture
Design
In vitro laboratory study
Authors
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TCID50 assay in BHK-21 cells supports FMD vaccine quality control; leaves open standardization across serotypes.
BHK-21 monolayer cells cultured in a roller system yield higher FMDV biological titers than suspension cells in a bioreactor, though suspension culture allows for larger-scale production.
Arju et al. (2022) studied Foot and mouth disease. BHK-21 monolayer cell culture (roller bottle) vs. BHK-21 clone 13 suspension cell culture (bioreactor) was evaluated on Biological titer (TCID50) of FMD virus serotypes. BHK-21 monolayer cell culture in roller bottles yielded higher biological titers (TCID50 up to 10^-9.83/ml) for FMD virus serotypes O, A, and Asia-1 compared to suspension cells.
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