On the surface structure of murine ascites tumors. I. Interactions with various phytoagglutinins

Phytoagglutinins originating from various plants agglutinated murine tumor cells and erythrocytes to varying degrees; some strongly, some moderately, and some not at all. The agglutinability of Yoshida sarcoma cells (rat ascites tumor) by various phytoagglutinins was essentially similar to that of ascites hepatoma 66 cells (rat ascites tumor), and similarity of agglutinability was also found between Ehrlich ascites carcinoma cells and leukemia 1210 cells, both of which were of murine origin. Since the phytoagglutinins react with the corresponding binding sites of cell surfaces in a similar manner to antibodies, the binding sites could be specified by the inhibition of the bindings after the addition of mono‐ or disaccharides. The treatment of Yoshida sarcoma cells with chemical reagents, hydrolytic enzymes or heat changed their agglutinability against the agglutinins. The extent to which their agglutinability was changed by these treatments, however, differed according to the kind of agglutinin used.