Ultramicroscopic carious changes were studied by using longspan ultrathin sections of dentin, which included a continuous piece of dentin from the pulp chamber wall up to the carious cavity floor. The changes observed in the inner carious dentin were gradual and continuous, with dissolution of crystals attached to the matrix fibers, whereas the changes observed in the outer carious dentin were rather abrupt and definite, with irregularly-scattered fine-granular crystals.
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Shimizu et al. (1981) studied this question.
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