Why the study?
Vaccine-derived poliovirus has become a serious issue, and the World Health Organization Global Action Plan requires stringent control of potentially poliovirus-infected materials.
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Generation of a poliovirus-nonsusceptible Vero cell line provides a safe platform for isolating emerging viruses without the risk of generating potentially poliovirus-infected materials.
Polio or poliomyelitis is a disabling and life-threatening disease caused by poliovirus (PV). As a consequence of global polio vaccination efforts, wild PV serotype 2 has been eradicated, and wild PV serotypes 1- and 3-transmitted cases have been largely eliminated except for in limited regions around the world. However, vaccine-derived PV, pathogenically reverted live PV vaccine strains in vaccinated humans, has become a serious issue. For the global eradication of polio, the World Health Organization is conducting the third edition of the Global Action Plan, which is requesting stringent control of potentially PV-infected materials. To facilitate the mission, we generated a PV-nonsusceptible Vero cell subline, which may serve as an ideal replacement of standard Vero cells to isolate emerging/re-emerging viruses without the risk of generating PV-infected materials.
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Okemoto‐Nakamura et al. (2020) studied Poliovirus infection (in vitro). CRISPR/Cas9 disruption of PVR1 and PVR2 genes vs. Parental Vero cells was evaluated on Poliovirus replication and viral RNA synthesis.
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