Analytical validation study reveals elevated phytoene levels in Rubybell tomato extract, highlighting its utility for functional food standardization.
In this study, the lycopene and phytoene contents in commercial tomato (Solanum lycopersicum L.) extracts and a newly developed ‘Rubybell’ extract were compared using a validated high-performance liquid chromatography with diode-array detection method. Phytoene and lycopene were selected as key targets because ‘Rubybell’ was bred for enhanced functional carotenoids, and phytoene is increasingly valued for its bioavailability and skin-health potential. The method showed excellent linearity (R2>0.999), with limit of detection/limit of quantitation values of 0.0668/0.2024 and 0.0604/0.1830 μg/mL for phytoene and lycopene, respectively. Precision relative standard deviations (RSDs) were within 6.16%, and the recovery ranged from 84.2 to 102.4% with RSDs below 2.8%. PhytoflORALTM showed the highest lycopene content, whereas ‘Rubybell’ had the highest phytoene content. This method is suitable for standardizing tomato-derived functional materials.
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Moon et al. (2026) studied this question.
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