What does this research mean for the field?

Cationic dextran nanoparticles synthesized via reductive amination efficiently condense pDNA, exhibit low cytotoxicity, and achieve high cellular uptake in vitro, serving as a viable non-viral vector for gene delivery. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To synthesize, characterize, and evaluate a cationic dextran derivative as a safe, efficient non-viral carrier for plasmid DNA delivery.

September 3, 2026Advanced Pharmaceutical BulletinOpen Access

Development of Cationic Dextran Nanoparticles via Reductive Amination: Enhanced Cellular Uptake and Low Cytotoxicity in Gene Transfection

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Authors

KKKianoosh KaramiPayame Noor University SUSeyed Sepehr UroomiyeHamedan University of Medical Sciences KDKatayoun DerakhshandehHamedan University of Medical Sciences

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Implication

In vitro study demonstrates high cellular uptake and low cytotoxicity of cationic dextran nanoparticles in NIH3T3 cells, indicating a promising platform for non-viral gene delivery.

Key Points

To synthesize, characterize, and evaluate a cationic dextran derivative as a safe, efficient non-viral carrier for plasmid DNA delivery.
Synthesized cationic dextran via periodate oxidation and reductive amination with 1,6-hexamethylenediamine, verifying structure using 1H NMR and FT-IR spectroscopy.
Assembled nanoparticles with pDNA and characterized hydrodynamic size, zeta potential via dynamic light scattering, and morphology using scanning electron microscopy.
Assessed buffering capacity, pDNA condensation, DNase I protection, cytotoxicity via MTT assay, and cellular uptake via flow cytometry in NIH3T3 cells.
Nanoparticles displayed uniform diameters ranging from 207 to 245 nm and positive zeta potentials between +57.2 and +73.7 mV.
The polymer exhibited strong buffering capacity within the endosomal pH range, efficiently condensed pDNA, and conferred partial protection against DNase I degradation.
Polyplexes demonstrated low cytotoxicity at 24 and 48 hours, with flow cytometry confirming internal uptake in over 95% of NIH3T3 cells.

Cite This Study

Karami et al. (2026) studied this question.

synapsesocial.com/papers/6a9935b1636c6408cfa7e1bb https://doi.org/10.34172/apb.46469

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Implication

In vitro study demonstrates high cellular uptake and low cytotoxicity of cationic dextran nanoparticles in NIH3T3 cells, indicating a promising platform for non-viral gene delivery.

Development of Cationic Dextran Nanoparticles via Reductive Amination: Enhanced Cellular Uptake and Low Cytotoxicity in Gene Transfection

Discussion

Member takes

Implication

Key Points

Cite This Study

Development of Cationic Dextran Nanoparticles via Reductive Amination: Enhanced Cellular Uptake and Low Cytotoxicity in Gene Transfection

Discussion

Member takes

Implication

Key Points

Cite This Study

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