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The advantage in using membrane filters for crosses between P+(donor) and P−(recipient) strains ofVibrio cholerae(Bhaskaran, 1964; Bhaskaran, Sinha & Iyer, 1967) is probably due to more frequent and effective contacts between the cells on the surface of the membrane than is possible in fluid media with actively motile cells of this species. The transfer of the fertility (P) factor itself, as well as the yield of genetic recombinants, was considerably improved by this procedure.
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Iyer et al. (1969) studied this question.
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