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A satisfactory tube antigen was produced from isolate WVU 1853 Mycoplasma synoviae grown in a modification of mycoplasma broth formulated by Frey. The tube antigen, employed in the tube test, showed good specificity when tested with 187 known M.-synoviae-positive serums, 60 known M.-gallisepticum-positive, and 30 normal. The serums were confirmed by HI test as being positive or negative to M. synoviae or M. gallisepticum.
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Vardaman et al. (1971) studied this question.
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