Key result
An RT-PCR procedure with amplification inhibitor removal detected enteroviruses in all isolation-positive shellfish samples and identified additional positive samples missed by conventional isolation.
Population
Molluscan shellfish (bivalve shellfish) naturally polluted field samples
Comparison
Reverse transcription-PCR with a procedure for… vs Conventional enterovirus isolation
Design
Preclinical
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May enhance enterovirus detection in shellfish; leaves open validation for clinical or regulatory use.
The RT-PCR procedure with inhibitor removal effectively detects enteroviruses in polluted shellfish, demonstrating potentially higher sensitivity than conventional isolation.
A 1995 study studied Enteric virus contamination in shellfish. RT-PCR with amplification inhibitor removal vs. Conventional enterovirus isolation was evaluated on Detection of enteroviruses and hepatitis A virus. An RT-PCR procedure with amplification inhibitor removal detected enteroviruses in all isolation-positive shellfish samples and identified additional positive samples missed by conventional isolation.
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