Key result
The micro ELISA showed excellent correlation with IFA and CF tests, detecting T. cruzi antibodies in 29.5% of El Salvador army recruits compared to 29.5% by IFA and 31.5% by CF.
Why the study?
Does micro ELISA accurately detect antibodies to Trypanosoma cruzi compared to IFA and CF tests in human serum samples?
Population
369 serum samples, including 50 from residents of the southeastern United States, 17 from hospitalized…
Comparison
Micro enzyme-linked immunosorbent assay for… vs Complement fixation test and indirect…
Design
Cross-sectional
Authors
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May support micro ELISA for Chagas serodiagnosis in limited settings; leaves open prospective validation before wider adoption.
Cross-Sectional (n=369)
Does micro ELISA accurately detect antibodies to Trypanosoma cruzi compared to IFA and CF tests in human serum samples?
Absolute Event Rate: 29.5% vs 31.5%
Micro ELISA demonstrates excellent correlation with IFA and CF tests, suggesting it is a promising and practical tool for serodiagnosis and seroepidemiology of Chagas' disease.
Spencer et al. (1980) conducted a cross-sectional in Trypanosoma cruzi infection (Chagas' disease) (n=369). Micro enzyme-linked immunosorbent assay (ELISA) vs. Complement fixation test (CF) and indirect fluorescent antibody test (IFA) was evaluated on Detection of antibodies to T. cruzi in El Salvador army recruits. The micro ELISA showed excellent correlation with IFA and CF tests, detecting T. cruzi antibodies in 29.5% of El Salvador army recruits compared to 29.5% by IFA and 31.5% by CF.