Key result
Whole protein sequencing of three rotavirus isolates from Chinese calves identified two species A strains and one species B strain, demonstrating specific genotype constellations and viral evolution.
Why the study?
Rotaviruses account for severe diarrhea in children and young animals globally.
The study successfully isolated and characterized species A and B bovine rotaviruses from Chinese calves, providing insights into viral evolution and genotype constellations.
Characterizes bovine rotavirus diversity in Chinese calves; leaves open zoonotic risks and vaccine implications pending further study.
Rotaviruses (RVs) account for severe diarrhea in children and young animals globally. In the current study, the fecal samples of diarrheic calves from a beef farm in Inner Mongolia were screened for RVA by ELISA and RT-PCR, followed by culture of three positive RVA samples in the MA-104 cell line. After 10 blind passages, cytopathic effects (CPE) appeared as detachment, granulation, and clustering of the inoculated cells. The virus isolates were identified by RT-PCR (VP6 gene RVA) and ESI-LC-MS/MS for whole protein sequencing. The protein sequences demonstrated the presence of two strains from species A rotavirus and one RVB strain; RVA/Cow-tc/CHN/35333/2019/G6P[5] was mixed with one RVB strain (RVB/Cow-tc/CHN/35334/2019/G5P[3]) in two samples, and RVA/Cow-tc/CHN/10927/2019/G8P[7] was found in one sample. They are of genotype constellations (G6-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3), (G8-P[7]-I5-R1-C1- M2-A1-N1-T1-E1-H1), and (G5-P[3]-I3-R5-C5-A5-N4-H5), respectively. Besides, phylogenetic analysis of the obtained sequences demonstrated viral evolution.
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Elkady et al. (2021) studied Rotavirus infection in calves (n=3). Viral isolation and whole protein sequencing was evaluated on Identification and characterization of rotavirus strains. Whole protein sequencing of three rotavirus isolates from Chinese calves identified two species A strains and one species B strain, demonstrating specific genotype constellations and viral evolution.
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