Key result
Both inhibition of glycolysis and enhancement of perfusate H+ buffering capacity before ischaemia attenuated myocardial stunning, but their protective effect was lost when combined.
Why the study?
Does inhibition of glycolysis or increased perfusate H+ buffering capacity, alone or in combination, reduce myocardial stunning in isolated rat hearts?
Population
Hearts of male Sprague-Dawley rats subjected to 15 min of total ischaemia and 30 min of reperfusion
Comparison
Preperfusion for 10 min with oxygenated or… vs Comparison between individual interventions and…
Design
Preclinical
Follow-up
30 min of reperfusion
Authors
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Should not yet change clinical cardioprotection; leaves open mechanistic interactions of glycolysis inhibition and H+ buffering in ischemia-reperfusion models.
Does inhibition of glycolysis or increased perfusate H+ buffering capacity, alone or in combination, reduce myocardial stunning in isolated rat hearts?
Inhibition of glycolysis or increased H+ buffering capacity individually attenuates myocardial stunning, but their combination paradoxically worsens functional recovery and Ca2+ overload.
Tani et al. (1993) studied Myocardial stunning. Inhibition of glycolysis (2-deoxyglucose) or enhancement of H+ buffering capacity (HEPES) vs. Oxygenated or hypoxic buffer with 100 mM sucrose was evaluated on Myocardial stunning and functional recovery. Both inhibition of glycolysis and enhancement of perfusate H+ buffering capacity before ischaemia attenuated myocardial stunning, but their protective effect was lost when combined.
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