Key result
RNA extracted from archived rotavirus rapid test strips successfully identified 92% of the G genotypes found in corresponding stool suspensions, enabling molecular surveillance from stored diagnostic strips.
Observational (n=61)
No
Archived rotavirus rapid test strips stored at room temperature for up to 5 years can yield high-quality RNA for accurate genotyping and molecular epidemiology.
May enable retrospective rotavirus genotyping from stored strips; hypothesis-generating for expanded molecular surveillance.
Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005-06 rotavirus season. Accurate molecular epidemiologic data were recovered from affinity-concentrated rotavirus immobilized in rotavirus-positive bands from air-dried, diagnostic rotavirus rapid test strips (dipstick) stored at room temperature from 1 week to 5 years. G genotypes were identical for 21 paired dipsticks and suspensions, whereas dipsticks or suspensions detected an additional G genotype in 2 samples. RNA sequences from 7 pairs were identical. Phylogenetic analysis suggested previously unreported G2 sublineages and G9 lineages. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can reverse increasing difficulties in obtaining geographically representative stool samples and expand surveillance to regions lacking adequate laboratory facilities.
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Shulman et al. (2010) conducted an observational in Rotavirus gastroenteritis (n=61). RNA extraction from archived rotavirus rapid test strips vs. RNA extraction from saline stool suspensions was evaluated on Concordance of rotavirus G genotypes between dipsticks and stool suspensions. RNA extracted from archived rotavirus rapid test strips successfully identified 92% of the G genotypes found in corresponding stool suspensions, enabling molecular surveillance from stored diagnostic strips.
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