November 30, 2004Molecular and Cellular Biology

Sp3 Proteins Negatively Regulate β Myosin Heavy Chain Gene Expression during Skeletal Muscle Inactivity

Q: What is the clinical evidence from this study?

Study design: Other. Population: Skeletal muscle inactivity. Intervention: Non-weight-bearing (NWB) conditions vs. Control weight-bearing conditions. Primary outcome: βMyHC gene expression and Sp protein binding.

View Full Paper

Ask AI

Bookmark

Key result

Increased binding of Sp3 to GC-rich elements in the βMyHC promoter is a critical event in the down-regulation of βMyHC gene expression under non-weight-bearing conditions.

Population

Adult skeletal muscle (plantaris muscle), Drosophila SL-2 cells, and mouse C2C12 myotubes

Design

Preclinical

Authors

GTGretchen L. TsikaUniversity of Illinois Urbana-Champaign JJJuan JiWuhan University of Technology RTR. W. TsikaMissouri College

Discussion

Member takes

Implication

Does not inform clinical management of disuse atrophy; leaves open Sp3 as a mechanistic target for future human studies.

Structured PICO

Population

In vitro and in vivo animal models (rats, mice, and cell lines) studying the transcriptional regulation of skeletal muscle during inactivity and mechanical overload.

Exposure

Muscle inactivity (non-weight-bearing conditions) and mechanical overload

Outcome

beta myosin heavy chain (betaMyHC) gene expression and binding of Sp family proteins (Sp1, Sp3, Sp4) to the betaMyHC promotersurrogate

Sp3 proteins competitively inhibit Sp1 to down-regulate beta myosin heavy chain gene expression during skeletal muscle inactivity.

Limitations

Adult-stage muscle phenotypes and the effects of altered muscle loading conditions cannot be adequately duplicated in cultured muscle cells.

Cite This Study

Tsika et al. (2004) studied Skeletal muscle inactivity. Non-weight-bearing (NWB) conditions vs. Control weight-bearing conditions was evaluated on βMyHC gene expression and Sp protein binding. Increased binding of Sp3 to GC-rich elements in the βMyHC promoter is a critical event in the down-regulation of βMyHC gene expression under non-weight-bearing conditions.

synapsesocial.com/papers/6a99c977620b1fcd22fa4f55 https://doi.org/10.1128/mcb.24.24.10777-10791.2004

View Full Paper

Ask AI

Bookmark

Sp3 Proteins Negatively Regulate β Myosin Heavy Chain Gene Expression during Skeletal Muscle Inactivity

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Sp3 Proteins Negatively Regulate β Myosin Heavy Chain Gene Expression during Skeletal Muscle Inactivity

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider