April 1, 2001Cardiovascular ResearchOpen Access

Identification of novel, cardiac-restricted transcription factors binding to a CACC-box within the human cardiac troponin I promoter

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Population

Primary neonatal rat cardiac myocytes in culture, and human and rat cardiac muscle extracts

Design

Preclinical

Authors

Discussion

Does not alter clinical practice; leaves open the functional roles of HCB1/HCB2 in human cardiac gene regulation.

Population

Primary neonatal rat cardiac myocytes in culture, and human and rat cardiac muscle extracts

Intervention

Electrophoretic mobility shift assays (EMSA), transient transfection, and site-directed mutagenesis of the CACC-box/Sp1 consensus element

Outcome

Identification of factors binding to the CACC-box element and their functional importance in hTnIc promoter transcriptional activitysurrogate

Identifies novel heart-specific transcription factors (HCB1 and HCB2) essential for the expression of the human cardiac troponin I gene.

Kimberley A. Dellow (2001) studied this question.

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