Key result
A 41-bp fragment containing the MEF-2-like motif is essential for minimal cardiac-specific expression of the rat cTnT gene, involving cardiac-specific DNA-binding proteins of 22-26 kDa.
Population
Primary cultures of neonatal cardiomyocytes and cardiac fibroblasts, and protein extracts from rat hearts…
Design
Preclinical
Authors
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This motif may guide cardiac-specific promoter design; leaves open validation in human cells and therapeutic translation.
Identifies key cis-regulating elements and trans-activating factors, including a 41-bp fragment with an MEF-2-like motif, essential for cardiac-specific expression of the rat cTnT gene.
Wang et al. (1994) studied Transcriptional regulation of the rat cardiac troponin T gene. Chimeric genes (cTnT upstream region and CAT gene) was evaluated on Cardiac-specific gene expression. A 41-bp fragment containing the MEF-2-like motif is essential for minimal cardiac-specific expression of the rat cTnT gene, involving cardiac-specific DNA-binding proteins of 22-26 kDa.
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