March 1, 1997Biochemical JournalOpen Access

Regulation of the rat cardiac troponin I gene by the transcription factor GATA-4

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Population

In vitro models (cardiocytes and non-cardiac cells) and cloned rat cardiac troponin I gene

Comparison

Co-transfection of GATA-4 with troponin… vs Non-transfected cells, unmutated constructs, or…

Design

Preclinical

Authors

Discussion

Should not guide clinical care; leaves open GATA-4's in vivo role in human cardiac gene regulation.

Population

In vitro models (cardiocytes and non-cardiac cells) and cloned rat cardiac troponin I gene

Intervention

Co-transfection of GATA-4 with troponin I-luciferase constructs; mutation of proximal GATA-4 DNA-binding site

Comparator

Non-transfected cells, unmutated constructs, or different cell types

Outcome

Luciferase activity (gene expression) and specific binding of GATA-4 to oligonucleotidessurrogate

The transcription factor GATA-4 regulates the cardiac-specific expression of the rat cardiac troponin I gene in vitro.

Murphy et al. (1997) studied this question.

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