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September 1, 1987Molecular and Cellular Biology

Complex Regulation of the Muscle-Specific Contractile Protein (Troponin I) Gene

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Population

Mouse myogenic cell line stably transfected with a cloned quail troponin I contractile protein gene

Comparison

Deletion mutagenesis analyses vs Native intragenic site / wild-type gene…

Design

Preclinical

Authors

SKStephen F. KoniecznyCECharles P. Emerson

Discussion

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Overview

Does not change clinical practice; leaves open whether these elements regulate troponin I in human cardiac muscle.

Structured PICO

P
Population
Mouse myogenic cell line stably transfected with a cloned quail troponin I contractile protein gene
I
Intervention
Deletion mutagenesis analyses (moving and inverting regulatory elements)
C
Comparator
Native intragenic site / wild-type gene configuration
O
Outcome
Developmental expression measured by mRNA accumulation and nuclear runoff transcription assayssurrogate

The troponin I gene requires two distinct cis regulatory elements (one 5' flanking, one intragenic) for its developmental expression and massive transcriptional activation during myoblast differentiation.

Cite This Study

Konieczny et al. (1987) studied this question.

synapsesocial.com/papers/6a87c52e6e1dca10b7bb58c5https://doi.org/10.1128/mcb.7.9.3065-3075.1987
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem.1984 · 328 citations
  2. 2Fine Structure Genetic Analysis of a β-Globin Promoter1986 · 472 citations
  3. 3The Complete Sequence of the Mouse Skeletal α-Actin Gene Reveals Several Conserved and Inverted Repeat Sequences Outside of the Protein-Coding Region1986 · 96 citations
  4. 4Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.1984 · 344 citations
  5. 5The complete sequence of the chicken α-cardiac actin gene: a highly conserved vertebrate gene1985 · 73 citations