Key result
In STZ-induced diabetic mice, 10 miRNAs were up-regulated and 6 were down-regulated in heart tissue, potentially mediating cardiac hypertrophy and myocardial fibrosis via target genes such as RASA1, RAC1, TGFB3, and COL1A1.
Why the study?
Does STZ-induced diabetes alter miRNA expression and target genes related to cardiac hypertrophy and fibrosis in mice?
Does STZ-induced diabetes alter miRNA expression and target genes related to cardiac hypertrophy and fibrosis in mice?
p-value: p=<0.05
STZ-induced diabetic cardiomyopathy in mice is associated with differential expression of specific miRNAs and their target genes related to cardiac hypertrophy and fibrosis.
No practice change from mouse data; leaves open miRNA roles in diabetic cardiomyopathy for human validation.
Persistent hyperglycemia in diabetic patients has been associated with cardiac hypertrophy, myocardial fibrosis and cardiac dysfunction. However, the underlying mechanisms of this association have yet to be fully elucidated. The aim of this study was to investigate the expression and function of microRNAs (miRNAs) in diabetic cardiomyopathy. miRNA expression profiles were examined by miRNA microarray analysis in heart tissue from streptozotocin (STZ)-induced diabetic mice and non-diabetic mice. The targets of the altered miRNAs were predicted using the Sanger database. Then, the targets RASA1, RAC1, TGFB3 and COL1A1, related to cardiac hypertrophy or myocardial fibrosis, were selected to analyze the miRNA level by real-time reverse transcription (RT-PCR). Gene Ontology (GO) was further applied to describe the function of each miRNA target gene and to elucidate their combined effects in diabetic cardiomyopathy. Up-regulated (n=10) and down-regulated (n=6) miRNAs were identified in diabetic cardiomyopathy. Target genes (n=192) were pooled from the Sanger database. Among the 192 targets, the mRNA expression of RASA1, RAC1, TGFB3 and COL1A1 was increased in diabetic cardiomyopathy. Thirty one GO functions were enriched in diabetic cardiomyopathy. These results demonstrate that miRNAs may mediate cardiac hypertrophy and myocardial fibrosis in diabetic cardiomyopathy via their targets, and provide insights into the pathogenesis of diabetic cardiomyopathy.
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A 2011 study studied Diabetic cardiomyopathy (n=25). Streptozotocin (STZ) vs. Citrate buffer was evaluated on Differentially expressed miRNAs in heart tissue (p=<0.05). In STZ-induced diabetic mice, 10 miRNAs were up-regulated and 6 were down-regulated in heart tissue, potentially mediating cardiac hypertrophy and myocardial fibrosis via target genes such as RASA1, RAC1, TGFB3, and COL1A1.
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