Sodium, potassium and magnesium chloride, at 1.25, 2.50 and 5.00%, were studied using differential scanning calorimetry. Increasing NaCl resulted in decrease in myosin transition temperature (T 1 , first transition) and enthalpy (H 1 ); both showed significant (P<0.05) linear response. The sarcoplasmic proteins and collagen denaturation temperature (T 2 , second transition) increased significantly over the control when 1.25% NaCl was added, but were the same as control when 2.5% and 5.0% were used. Actin denaturation temperature (T 3 , third transition) tended to increase when NaCl was added, but enthalpy decreased. Potassium chloride showed similar effects to NaCl on T 1 , T 2 , H 1 and H 3 . Increasing magnesium chloride had much more pronounced effect on decreasing second enthalpy compared to the monovalent salts. At 5% MgCl 2 T 1 increased significantly.
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Barbut et al. (1991) studied this question.