Key result
There was no significant difference in cell oxygen consumption or cytochrome-c oxidase activity between cybrids containing mtDNA from elite endurance athletes and those from sedentary controls.
Why the study?
Does mitochondrial DNA from endurance athletes improve mitochondrial respiratory function compared to sedentary controls in a cybrid cell model?
Observational (n=11)
Does mitochondrial DNA from endurance athletes improve mitochondrial respiratory function compared to sedentary controls in a cybrid cell model?
The OXPHOS enzymes coded by mtDNA do not strongly influence whole-body aerobic fitness, suggesting that the higher muscle enzyme activity observed in endurance athletes is not due to mtDNA polymorphism.
Answers are grounded in this paper and cite their sources.
mtDNA variants unlikely to drive endurance phenotypes; leaves open nuclear DNA, training, and epigenetic contributions for targeted human studies.
The purpose of this study was to examine the relation between whole-body aerobic capacity and mitochondrial facilities. The mitochondrial enzyme system of oxidative phosphorylation (OXPHOS) is encoded both by mitochondrial DNA (mtDNA) and nuclear DNA. To identify the effect of mtDNA on whole-body aerobic capacity, we fused the platelets of the study subjects that contained mtDNA but that lacked nuclear DNA with rho(0) HeLa cells, which lacked mtDNA, and isolated repopulated cybrids. The mitochondrial respiratory functions of the cybrids, estimated from cell oxygen consumption and cytochrome-c oxidase (CCOX), were compared between endurance athletes and sedentary controls. The oxygen consumption was 18.5 +/- 3.9 and 18.2 +/- 4.1 nmol/min/ml/10(7) cells in athletes and controls, respectively. The CCOX activity was 98.8 +/- 17.5 and 116.7 +/- 9.8%, compared with fibroblasts in athletes and controls, respectively. No significant difference was noted between groups in either cell oxygen consumption or CCOX activity. These results show that the OXPHOS enzymes coded by mtDNA do not strongly influence whole-body aerobic fitness.
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Soma et al. (2001) conducted an observational in Healthy (n=11). mtDNA from endurance athletes vs. mtDNA from sedentary controls was evaluated on Cell oxygen consumption (nmol/min/ml/10^7 cells) (p=>0.05). There was no significant difference in cell oxygen consumption or cytochrome-c oxidase activity between cybrids containing mtDNA from elite endurance athletes and those from sedentary controls.
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