Key result
The pOXB dual-plasmid system significantly increased AAV vector productivity and the percentage of full capsids packaged compared to the pDG dual design and triple transfection.
Why the study?
AAV vectors are typically manufactured via triple transfection or older dual-plasmid systems, driving the development of platforms that improve vector productivity and packaging efficiency.
The pOXB dual-plasmid system improves AAV vector manufacturing productivity and packaging quality while maintaining the flexibility of transient transfection.
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May improve AAV manufacturing efficiency; leaves open translation to clinical-grade production and therapeutic use.
Lieshout et al. (2023) studied Adeno-associated virus (AAV) vector production. pOXB dual-plasmid system vs. pDG dual design and triple transfection was evaluated on AAV vector productivity and percentage of full capsids packaged. The pOXB dual-plasmid system significantly increased AAV vector productivity and the percentage of full capsids packaged compared to the pDG dual design and triple transfection.
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