Key result
RT-PCR assays identified rotavirus infection in 71.5% (103 of 144) of diarrheic pig fecal samples that previously had inconclusive results by ss-PAGE, revealing high rates of atypical rotaviruses B and C.
Cross-Sectional (n=144)
Yes
RT-PCR is highly sensitive for detecting atypical rotaviruses B and C in porcine fecal samples that yield inconclusive results by standard ss-PAGE.
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RT-PCR may aid detection of atypical rotaviruses in porcine diarrhea; hypothesis-generating and requires prospective validation before changing practice.
Médici et al. (2011) conducted a cross-sectional in Neonatal diarrhea in pigs (porcine rotavirus infection) (n=144). Reverse transcription-polymerase chain reaction (RT-PCR) vs. Silver-stained polyacrylamide gel electrophoresis (ss-PAGE) was evaluated on Detection of rotavirus infection (groups A, B, and C). RT-PCR assays identified rotavirus infection in 71.5% (103 of 144) of diarrheic pig fecal samples that previously had inconclusive results by ss-PAGE, revealing high rates of atypical rotaviruses B and C.
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