Key result
Rotavirus was demonstrated by electron microscopy in 27 of 195 samples and by ELISA in 44 samples, with successful adaptation of three group A isolates to cell culture and identification of one group C rotavirus.
The study successfully isolated and adapted porcine group A rotaviruses to cell culture and identified a group C rotavirus strain in a Czech swine herd.
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Provides swine rotavirus isolates for lab studies; leaves open veterinary or zoonotic applications.
Smítalová et al. (2006) studied Rotavirus infection (diarrhea) (n=195). Laboratory analysis (Rotavirus isolation and characterization) was evaluated on Demonstration and isolation of rotavirus. Rotavirus was demonstrated by electron microscopy in 27 of 195 samples and by ELISA in 44 samples, with successful adaptation of three group A isolates to cell culture and identification of one group C rotavirus.
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