Key result
Rapamycin did not inhibit IGF-II-induced proliferation or differentiation of rat, mouse, or human skeletal muscle cells, indicating p70 S6 kinase is not required for this process.
Population
Rat L6E9 myoblasts, mouse Sol8 myoblasts, and human myoblasts derived from skeletal muscle biopsies
Comparison
Rapamycin (1 to 20 ng/ml) and LY294002 vs Control (absence of inhibitors)
Design
Preclinical
Authors
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p70 S6 kinase is dispensable for IGF-II myogenesis; leaves open PI3K effector mechanisms in skeletal muscle differentiation.
p70 S6 kinase activation is not required for IGF-induced differentiation of skeletal muscle cells, whereas PI 3-kinase is essential.
Canicio et al. (1998) studied Skeletal muscle cell differentiation. Rapamycin vs. LY294002 or no rapamycin was evaluated on IGF-II-induced myoblast proliferation and differentiation (myotube formation and muscle-specific marker expression). Rapamycin did not inhibit IGF-II-induced proliferation or differentiation of rat, mouse, or human skeletal muscle cells, indicating p70 S6 kinase is not required for this process.
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