Determination of Dissociation Constants of Complicated Compounds by Capillary Zone Electrophoresis

In this work, the whole theoretical methods for the determination of pKa1. and pKa2. of complicated compounds are proposed by capillary zone electrophoresis. The pKa values are achieved by non‐linear regression analysis by taking into consideration the effect of activity coefficient. This is the first report on determining the dissociation constants of gastrodin, magnolol, honokiol, quercetin, curcumin, diethylstilbestrol, 4‐acetamidophenol, eugenol and paeonol.