Key result
The 30K protein encoded by MHV-A59 ORF 2a is localized primarily in the cytosol of infected mouse fibroblasts, consistent with a potential role as an RNA binding protein.
The MHV-A59 ORF 2a encodes a 30K protein localized in the cytosol, consistent with a potential role as an RNA binding protein.
Hypothesis-generating for MHV accessory protein function; leaves open roles in replication and requires validation beyond fibroblasts.
A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17Cl-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.
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Zoltick et al. (1990) studied Mouse hepatitis virus (MHV-A59) infection. cDNA vector encoding a bacterial/viral fusion protein of MHV-A59 ORF 2a was evaluated on Protein localization. The 30K protein encoded by MHV-A59 ORF 2a is localized primarily in the cytosol of infected mouse fibroblasts, consistent with a potential role as an RNA binding protein.
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