Key result
Translation of MHV virion RNA in a cell-free system produces a large polyprotein that is rapidly cleaved into smaller proteins, a process inhibited by leupeptin and ZnCl2.
MHV virion RNA is translated into a large polyprotein that is subsequently cleaved into smaller functional proteins, contrasting with the minimal proteolytic processing of other MHV proteins.
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No clinical implications; leaves open whether polyprotein cleavage is a viable antiviral target in vivo.
Denison et al. (1986) studied Mouse hepatitis virus (MHV-A59) infection. Protease inhibitors (leupeptin and ZnCl2) vs. Absence of inhibitors was evaluated on Polypeptide products of cell-free translation. Translation of MHV virion RNA in a cell-free system produces a large polyprotein that is rapidly cleaved into smaller proteins, a process inhibited by leupeptin and ZnCl2.
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