Key result
Viral or virus-induced factors are necessary for the MHV RNA-dependent RNA polymerase to associate with membranes of replication complexes, requiring a specific 38-amino-acid domain.
Population
Host cells infected with Mouse hepatitis virus (MHV) or expressing Gpol fusion proteins
Comparison
Cloning and expression of the pol domain of gene… vs Mock-infected cells
Design
Preclinical
Authors
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No immediate clinical application; leaves open conservation and targeting of the domain in human coronaviruses.
Viral or virus-induced factors are necessary for the MHV RNA-dependent RNA polymerase to associate with membranes of replication complexes, mediated by a specific 38-amino-acid domain.
Brockway et al. (2003) studied Mouse hepatitis virus (MHV) infection. Gpol expression and MHV infection vs. Mock-infected cells and Gpol deletion mutants was evaluated on Pol association with cellular membranes and replication complexes. Viral or virus-induced factors are necessary for the MHV RNA-dependent RNA polymerase to associate with membranes of replication complexes, requiring a specific 38-amino-acid domain.
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