Effective Treatment of Diabetic Cardiomyopathy and Heart Failure with Reconstituted HDL (Milano) in Mice

Figures

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  Figure 1. Time course of body weight (A) and blood glucose levels (B) in C57BL/6N mice fed the standard chow (SC) diet or the high-sugar/high-fat (HSHF) diet. Plasma insulin (C), free fatty acids (D), and adiponectin (E) levels at 16 weeks after the start of the diet. Week 0 in panels A and B corresponds to the age of 12 weeks, the start of the HSHF diet. All data represent mean ± SEM. (n = 15 for SC diet; n = 20 for HSHF diet).

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  Figure 2. Schematic representation of the study design.

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  Figure 3. Total cholesterol (A), non-HDL cholesterol (B), and HDL cholesterol plasma levels (C) and plasma triglyceride levels (D) in female C57BL/6N mice at the time of sacrifice. All data represent means ± SEM (n = 8).

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  Figure 4. Time course of body weight (A) and blood glucose levels (B) in reference, buffer, and MDCO-216 C57BL/6N mice fed the SC diet or the HSHF diet. Plasma insulin (C), free fatty acids (D), and adiponectin (E) levels at the time of sacrifice. Week 0 in panels A and B corresponds to the age of 12 weeks, the start of the HSHF diet. All data represent mean ± SEM (n = 15 for SC diet groups; n = 20 for HSHF diet groups).

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  Figure 5. Representative Sirius-red stained heart cross-sections of reference, buffer, and MDCO-216 SC diet and HSHF diet mice. Scale bar represents 1 mm.

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  Figure 6. Immunohistochemical and histochemical analysis of the myocardium of reference, buffer, and MDCO-216 SC diet and HSHF diet mice. Representative photomicrographs show laminin-stained cardiomyocytes, CD31-positive capillaries, Sirius-red-stained collagen, and 3-nitrotyrosin positive area. Scale bar represents 50 µm.

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  Figure 7. Lactate pre-exercise (A), distance covered (B), and lactate post-exercise (C) in reference, buffer, and MDCO-216 C57BL/6N mice fed the SC diet or the HSHF diet. All data represent means ± SEM (n = 10).

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  Figure 8. Plasma TNF-α concentration in the reference buffer, and MDCO-216 C57BL/6N mice fed the SC diet or the HSHF diet. All data represent means ± SEM (n = 10).