Complete Genome Sequence of Pseudomonas sp. Con_6, a Putative Novel Species Isolated from Agricultural Soil Collected in the Republic of Korea

The genus Pseudomonas (family Pseudomonadaceae) is among the most ecologically versatile bacterial genera, occupying soil, water, plant rhizospheres, and diverse host-associated environments, and characteristically encoding broad metabolic repertoires including siderophores, antibiotics, and secondary metabolites relevant to plant growth promotion and biocontrol [1].Strain Con_6 was recovered from agricultural soil in the Republic of Korea and provisionally identified as Pseudomonas sp. by 16S rRNA gene sequencing; wholegenome sequencing was undertaken to resolve its species identity and characterize its genomic features.Strain Con_6 was isolated from agricultural soil collected in Chilgok, Daegu, Republic of Korea (35°95′28.97″N,128°56′16.01″E).Soil was suspended in 0.85% (w/v) NaCl, serially diluted, and plated on tryptic soy agar (TSA; Difco, USA) at 37℃ for 24-48 h.A single colony was subcultured three times for purity and grown overnight in TSB at 37℃ and 200 rpm.Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, USA) with inversion mixing to preserve high-molecular-weight DNA for long-read sequencing.DNA quality was verified by NanoDrop and Qubit at the KNU NGS Core Facility (Republic of Korea).Whole-genome sequencing was performed on the Oxford Nanopore MinION platform using the Native Barcoding Kit V14 (SQK-NBD114-24) with an R10.4.1 flow cell.Read quality statistics summarized by NanoStat v1.6.0 yielded 38,514 reads (total 656.9 Mb; read length N50, 28,735 bp; mean quality, Q20.2; longest read, 169,787 bp).Low-quality reads and those shorter than 1,000 bp were removed with Filtlong v0.2.1, and the filtered reads were assembled de novo using Flye v2.9.5 [2] in --nano-hq mode with a target genome size of 6.5 Mb, with 3 rounds of internal polishing (-i 3); no additionalWe report the complete genome sequence of Pseudomonas sp.Con_6, a putative novel species isolated from agricultural soil in the Republic of Korea.The genome comprises a single circular chromosome of 7,034,194 bp (GC content, 63.43%).Phylogenomic analyses via ANI (91.945%) and dDDH (d4, 45.4%) against the closest type strain, Pseudomonas ulcerans, both fell below species demarcation thresholds, supporting novel species recognition.Functional annotation revealed 10 biosynthetic gene clusters including a pyoverdine siderophore locus, 99 carbohydrate-active enzymes, 4 antibiotic resistance genes, and 28 mobile genetic elements.