Complete Genome Sequence of Lysinibacillus xylanilyticus PK6, Isolated from Soybean Rhizosphere Soil in a Nitrogen-Deficient Field

The genus Lysinibacillus (family Caryophanaceae, phylum Bacillota) comprises Gram-positive, spore-forming, rod-shaped bacteria that are widely distributed in soil and the plant rhizosphere, where several members display plant growth-promoting and biocontrol activities [1].Lysinibacillus xylanilyticus was originally described as a xylan-degrading bacterium isolated from forest humus [2].Here we report the complete genome sequence of strain PK6, its taxonomic assignment, its plant-beneficial gene content and a preliminary genomebased safety screen relevant to its use as a rhizosphere inoculant.Strain PK6 was isolated from the rhizosphere soil of soybean (Glycine max (L.) Merr.) grown in a nitrogenomission field (phosphorus and potassium applied) maintained under a barley-soybean rotation for approximately 50 years at the Gyeongsangbuk-do Agriculture Center, Republic of Korea [3].A single colony was isolated on Luria-Bertani (LB) agar and cultured at 30℃.Preliminary identification by 16S rRNA gene sequence analysis assigned the isolate to the genus Lysinibacillus.Genomic DNA extraction and quality assessment were performed at KNU NGS Core Facility (Republic of Korea); DNA was extracted from a 12-h LB broth culture using the Wizard Genomic DNA Purification Kit (Promega, USA). Whole-genome sequencing on an ONT PromethIONLysinibacillus xylanilyticus PK6 is a Gram-positive, spore-forming bacterium isolated from soybean (Glycine max) rhizosphere soil in Gyeongsangbuk-do, Republic of Korea.Its complete genome comprises a single circular chromosome of 5,191,412 bp (36.9% GC) encoding 4,776 protein-coding genes, and average nucleotide identity supported its assignment to L. xylanilyticus (fastANI, 96.0%).The genome carries candidate genes for siderophore-mediated iron acquisition, including a putative partial nonribosomal peptide synthetase (NRPS)-independent siderophore (NIS)-like cluster, and for organic-phosphorus mineralization, but also a complete tripartite hemolysin BL homolog locus.The sequence provides a reference for the species and identifies beneficial and safety-relevant loci for experimental evaluation.