Key result
Blood samples collected in hirudin tubes yielded significantly higher area under the curve values for platelet aggregation across most agonists compared to sodium citrate tubes in healthy Koreans.
Why the study?
What are the reference intervals for Multiplate platelet function analyzer parameters in healthy Koreans, and how do hirudin and sodium citrate anticoagulants compare?
Cross-Sectional (n=35)
No
What are the reference intervals for Multiplate platelet function analyzer parameters in healthy Koreans, and how do hirudin and sodium citrate anticoagulants compare?
This study establishes basic reference intervals for the Multiplate platelet function analyzer in healthy Koreans, demonstrating that hirudin anticoagulation yields significantly higher and more stable AUC values than sodium citrate.
Answers are grounded in this paper and cite their sources.
Tube choice affects Multiplate reference values in Koreans; leaves open standardization for clinical use and other populations.
This article is available from http://www.labmedonline.org 2013, Laboratory Medicine Online This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Background: The Multiplate analyzer (Dynabyte GmbH) has been recently introduced as a platelet function test for patients taking antiplatelet drugs. The study aimed at providing basic data for determining the reference interval of parameters produced by Multiplate in Koreans and to study the factors that influence those parameters. Methods: Blood was collected from 35 healthy volunteers (female 18, male 17) into tubes containing hirudin or 3.2% sodium citrate. Whole blood platelet aggregations triggered by adenosine-5’-diphosphate (ADP), ADP-high sensitive (ADP+PGE1 only in hirudin samples), arachidonic acid (AA), collagen or thrombin receptor activator peptide (TRAP) were investigated using Multiplate according to the manufacturer’s instructions. Data from healthy volunteers for the area under the curve (AUC) were determined from the central 95th percentile of the results. Results: The values of AUC in hirudin samples for all agonists were significantly higher than those in sodium citrate samples. The AUC values in hirudin (sodium citrate) samples were as follows: ADP 38-107 (18-119) U; ADP+PGE1 16-91 U; AA 64-156 (32-117) U; collagen 53-112 (26-108) U; and TRAP 81-163 (49-149) U. The parameters from Multiplate were significantly correlated with leukocyte counts, but not with hematocrit levels. Conclusions: Although our data were derived from only 35 subjects, the results are expected to be helpful in determining the reference interval at a single institute and may serve as basic data for future cumulative data of reference intervals from multiple institutes in Korea.
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Baik et al. (2013) conducted a cross-sectional in Healthy volunteers (n=35). Hirudin blood collection tubes vs. 3.2% sodium citrate tubes was evaluated on Area under the curve (AUC) for platelet aggregation. Blood samples collected in hirudin tubes yielded significantly higher area under the curve values for platelet aggregation across most agonists compared to sodium citrate tubes in healthy Koreans.
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