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December 1, 2017Platelets

Considering any agonist, 35% and 67% of initially 'normal' responses became 'abnormal' at 120 min and 180 min, respectively, with up to 60% reduction in platelet counts at 240 min in hirudin blood.

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Why the study?

Does the time from blood collection affect platelet aggregation results using the Multiplate analyzer with hirudin tubes?

Population

21 consecutive blood samples collected into manufacturer supplied hirudin tubes

Comparison

Platelet function testing using the Multiplate… vs EDTA blood and sodium citrate blood

Design

Other

Follow-up

up to 240 minutes post-collection

Authors

KCKent ChapmanEFEmmanuel J. Favaloro

Discussion

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Overview

May warrant limiting Multiplate hirudin testing to ≤120 min to avoid artifactual results; leaves open validation of time-specific protocols.

Structured PICO

Does the time from blood collection affect platelet aggregation results using the Multiplate analyzer with hirudin tubes?

P
Population
21 consecutive blood samples collected into manufacturer supplied hirudin tubes
I
Intervention
Platelet function testing using the Multiplate analyzer at several time-points post-sample collection within the recommended 0.5-3 hour window
C
Comparator
EDTA blood (reference for platelet counts) and sodium citrate blood
O
Outcome
Time-associated reduction in platelet aggregation for major agonists (ADP, arachidonic acid, TRAP, collagen, ristocetin)surrogate

Multiplate testing using hirudin tubes shows significant time-dependent reduction in platelet aggregation after 120 minutes, suggesting a narrower optimal testing window of 30-120 minutes compared to the manufacturer's recommended 0.5-3 hours.

Cite This Study

Chapman et al. (2017) studied this question.

synapsesocial.com/papers/6a83faf3a3cef8348e997e77https://doi.org/10.1080/09537104.2017.1384540
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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  3. 3Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers2020 · 23 citations
  4. 4Evaluation of platelet aggregometry in dogs using the M ultiplate platelet analyzer: impact of anticoagulant choice and assay duration2012 · 45 citations
  5. 5Effect of preanalytical time-delay on platelet function as measured by multiplate, PFA-100 and VerifyNow2016 · 12 citations