Key result
Passage of MM virus in BHK-21 cells produced a virus that replicated to high titers (200-300 PFU/cell) in L cells with complete cytopathic effects, compared to <1 PFU/cell for mouse-brain stock.
Passage of MM virus in BHK-21 cells adapts the virus to replicate efficiently in L cells with complete cytopathic effects.
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May facilitate lab propagation of MM virus; leaves open in vivo relevance and requires animal validation before broader use.
MM virus (mouse-brain stock) replicated to a limited extent in L cells without cytopathic effects; the average yield was less than 1 plaque-forming unit/cell. Passage in BHK-21 cells resulted in MM virus [MM(BHK)] which replicated to high titers (200 to 300 plaque-forming units/cell) in L cells with complete cytopathic effects. Appearance of mature MM(BHK) virus in L-cell cultures begins 4 hr after infection and is completed by 8 hr. Release of mature virus was slow (less than 1% at 8 hr) but was completed by 24 hr.
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Giron et al. (1969) studied MM virus infection. Passage in BHK-21 cells (MM(BHK) virus) vs. MM virus (mouse-brain stock) was evaluated on Viral replication yield in L cells. Passage of MM virus in BHK-21 cells produced a virus that replicated to high titers (200-300 PFU/cell) in L cells with complete cytopathic effects, compared to <1 PFU/cell for mouse-brain stock.
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