In vitro experiment reveals high antimicrobial and antibiofilm efficacy of biogenic silver nanoparticles, suggesting their utility for medical coatings.
Key Points
To synthesize silver nanoparticles via a rapid green approach using salicylic acid as a reducing and capping agent, and to evaluate their antibacterial and antibiofilm properties.
Synthesized salicylic acid-mediated silver nanoparticles (SA-AgNPs) and characterized them using UV-Vis, DLS, SEM-EDS, TEM, XRD, FTIR, and TGA-DSC.
Evaluated antimicrobial and antibiofilm efficacy against Gram-positive (MRSA, MRSE, S. aureus, C. acnes) and Gram-negative (E. coli) pathogens, measuring MICs, kill kinetics, biofilm disruption, and membrane permeability.
SA-AgNPs inhibited bacterial growth with MICs of 8 μg/mL (MRSE), 9 μg/mL (S. aureus), 8 μg/mL (C. acnes), 4 μg/mL (MRSA), and 6 μg/mL (E. coli).
At 32 μg/mL, SA-AgNPs achieved 99.9% bactericidal efficiency against E. coli within 4 h, MRSA within 12 h, and C. acnes within 16 h, while also inhibiting 95.61% of MRSA biofilm formation.