Isolation of Inflammatory Macrophages from Swim Bladder of Tilapia.

Detailed studies of exudate macrophage-mediated inflammatory response in fish have not been possible mainly due to incomplete purification of inflammatory macrophages. Many studies have demonstrated that neutrophils migrate to the site of inflammation before macrophages during the acute phase of inflammation1-3). As previously described in mammals4), neutrophils were found to be involved in the migration of macrophages to the inflammatory site. In this study, we elicited exudation of macrophages with injection of neutrophil lysate and isolated inflammatory macrophages at high purity in tilapia.