What question did this study set out to answer?

To characterize early lung tissue interactions and transcriptional inflammatory responses to Mycobacterium bovis and Mycobacterium tuberculosis infections across different cattle breeds.

July 9, 2021Frontiers in Veterinary ScienceOpen Access

Mycobacterial Infection of Precision-Cut Lung Slices Reveals Type 1 Interferon Pathway Is Locally Induced by Mycobacterium bovis but Not M. tuberculosis in a Cattle Breed

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Authors

ARAude Rémot FCFlorence Carreras ACAnthony Coupé

Discussion

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Overview

Ex vivo study reveals strain-specific type I interferon induction by Mycobacterium bovis in cattle lung slices, indicating a molecular driver of host tropism.

Key Points

To characterize early lung tissue interactions and transcriptional inflammatory responses to Mycobacterium bovis and Mycobacterium tuberculosis infections across different cattle breeds.
Developed an ex vivo precision-cut lung slice (PCLS) platform using lung tissue obtained from Blonde d'Aquitaine and Charolaise cattle breeds.
Infected lung slices with fluorescent Mycobacterium bovis (strains AF2122 and Mb3601) and Mycobacterium tuberculosis (strains H37Rv and BTB1558) to evaluate bacterial localization and inflammatory gene expression.
Fluorescence microscopy localized Mycobacterium bovis within the alveolar compartment, adjacent to or inside alveolar macrophages and in direct contact with pneumocytes.
Blonde d'Aquitaine lung slices exhibited strong induction of neutrophil and monocyte recruitment mediators and selective activation of the type I interferon pathway by M. bovis strains, whereas M. tuberculosis strains induced very low responses.
Charolaise cattle lung slices did not mount comparable recruitment signatures, demonstrating marked breed-dependent divergence in early mucosal immune responses.

Cite This Study

Rémot et al. (2021) studied this question.

synapsesocial.com/papers/6a9b4d2cc52075934cd75c28 https://doi.org/10.3389/fvets.2021.696525

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July 9, 2021Frontiers in Veterinary ScienceOpen Access

Mycobacterial Infection of Precision-Cut Lung Slices Reveals Type 1 Interferon Pathway Is Locally Induced by Mycobacterium bovis but Not M. tuberculosis in a Cattle Breed

View Full Paper

Ask AI

Bookmark

Authors

ARAude Rémot FCFlorence Carreras ACAnthony Coupé

Discussion

Member takes

Overview

Ex vivo study reveals strain-specific type I interferon induction by Mycobacterium bovis in cattle lung slices, indicating a molecular driver of host tropism.

Key Points

To characterize early lung tissue interactions and transcriptional inflammatory responses to Mycobacterium bovis and Mycobacterium tuberculosis infections across different cattle breeds.
Developed an ex vivo precision-cut lung slice (PCLS) platform using lung tissue obtained from Blonde d'Aquitaine and Charolaise cattle breeds.
Infected lung slices with fluorescent Mycobacterium bovis (strains AF2122 and Mb3601) and Mycobacterium tuberculosis (strains H37Rv and BTB1558) to evaluate bacterial localization and inflammatory gene expression.
Fluorescence microscopy localized Mycobacterium bovis within the alveolar compartment, adjacent to or inside alveolar macrophages and in direct contact with pneumocytes.
Blonde d'Aquitaine lung slices exhibited strong induction of neutrophil and monocyte recruitment mediators and selective activation of the type I interferon pathway by M. bovis strains, whereas M. tuberculosis strains induced very low responses.
Charolaise cattle lung slices did not mount comparable recruitment signatures, demonstrating marked breed-dependent divergence in early mucosal immune responses.

Cite This Study

Rémot et al. (2021) studied this question.

synapsesocial.com/papers/6a9b4d2cc52075934cd75c28 https://doi.org/10.3389/fvets.2021.696525

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