Key result
Coincubation of lipoprotein lipase and Type IV VLDL with HepG2 cells induced a 3-fold increment in cellular esterified cholesterol and a 7-fold increase in cellular triglycerides (p<0.005).
Population
HepG2 cells and J774 cells incubated with Type IV hypertriglyceridemic very low density lipoproteins
Comparison
Coincubation of bovine milk lipoprotein lipase… vs Type IV VLDL alone
Design
Preclinical
Follow-up
24 hours
Authors
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May promote hepatic lipid accumulation in Type IV hyperlipoproteinemia; hypothesis-generating and requires in vivo validation before clinical consideration.
Effect estimate: 3-fold and 7-fold increase
p-value: p=<0.005
Lipolytic remodeling of large Type IV VLDL by lipoprotein lipase is a prerequisite for their uptake and subsequent lipid accumulation in HepG2 cells, a process dependent on cell-secreted rather than VLDL-associated apoE.
Evans et al. (1992) studied Type IV hypertriglyceridemia. Coincubation of bovine milk lipoprotein lipase (LPL) and Type IV VLDL vs. VLDL alone was evaluated on Cellular esterified cholesterol mass and cellular triglyceride mass (3-fold and 7-fold increase, p=<0.005). Coincubation of lipoprotein lipase and Type IV VLDL with HepG2 cells induced a 3-fold increment in cellular esterified cholesterol and a 7-fold increase in cellular triglycerides (p<0.005).
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