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July 1, 1996Synapse

Quantitative RT-PCR distribution of serotonin 5-HT6 receptor mRNA in the central nervous system of control or 5,7-dihydroxytryptamine-treated rats

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Authors

CGCaroline G�rardInsermSMSalah El MestikawyDouglas Mental Health University InstituteCLCécile LebrandUniversity of Lausanne

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Implication

Controlled animal study reveals stable serotonin 5-HT6 receptor expression following chemical neurotoxic lesion in rats, suggesting the receptor does not act as an autoreceptor.

Key Points

  • To quantify the baseline regional distribution of serotonin 5-HT6 receptor mRNA across the rat central nervous system and determine whether selective serotoninergic lesions induce adaptive changes in receptor expression.
  • Measured 5-HT6 receptor mRNA across central and peripheral nervous tissues in control rats using competitive reverse transcription-polymerase chain reaction (RT-PCR).
  • Infused 5,7-dihydroxytryptamine into the anteroventral dorsal raphe region of nomifensine-pretreated rats to selectively destroy serotoninergic projections, evaluating tissue after 3 weeks.
  • In control rats, 5-HT6 mRNA expression was highest in the nucleus accumbens, olfactory tubercle, and striatum, with moderate levels in the cortex and spinal cord, and low levels in the raphe and cerebellum.
  • Treatment with 5,7-dihydroxytryptamine reduced serotonin transporter mRNA levels by 90% in the anterior raphe area, confirming extensive serotoninergic denervation.
  • Levels of 5-HT6 receptor mRNA in the anterior raphe, nucleus accumbens, striatum, and hippocampus showed no significant difference between 5,7-dihydroxytryptamine-treated rats and controls.

Cite This Study

G�rard et al. (1996) studied this question.

synapsesocial.com/papers/6a9b76f9cd9747862c3a0c42https://doi.org/10.1002/(sici)1098-2396(199607)23:3<164::aid-syn5>3.0.co;2-6
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