Key result
Dansylaziridine-labeled troponin-C and troponin-C demonstrated rapid calcium binding and removal at both low and high affinity sites, though the resulting conformational change at high affinity sites is slow.
This basic science study elucidates the differential kinetics of calcium binding and conformational changes at the high and low affinity sites of troponin-C.
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Distinguishes troponin-C binding kinetics from conformational changes; leaves open relevance to human myocardial calcium handling.
Iio et al. (1980) studied this question. Dansylaziridine-labeled troponin-C vs. Troponin-C was evaluated on Kinetic properties of calcium ion binding and removal reactions. Dansylaziridine-labeled troponin-C and troponin-C demonstrated rapid calcium binding and removal at both low and high affinity sites, though the resulting conformational change at high affinity sites is slow.
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