What question did this study set out to answer?

To compare the therapeutic angiogenic potential and vascular functionality of adeno-associated virus delivery of stabilized HIF-1α versus VEGF in skeletal muscle.

June 15, 2005The FASEB Journal

Stabilized HIF‐1α is superior to VEGF for angiogenesis in skeletal muscle via adeno‐associated virus gene transfer

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To compare the therapeutic angiogenic potential and vascular functionality of adeno-associated virus delivery of stabilized HIF-1α versus VEGF in skeletal muscle.
Injected recombinant adeno-associated viruses encoding stabilized HIF-1α, VEGF164, or VEGF120 into mouse tibialis anterior muscles.
Assessed capillary sprouting, endothelial proliferation, and vascular leakiness using immunohistochemistry and Evans Blue permeability assays.
Evaluated functional muscle perfusion with Doppler ultrasound imaging, including co-expression trials with angiopoietin-1 and platelet-derived growth factor-B.
Stabilized HIF-1α markedly increased both capillary sprouting and endothelial proliferation to form organized capillaries, whereas VEGF isoforms induced endothelial proliferation without proper capillary assembly.
Evans Blue assays showed that stabilized HIF-1α did not increase vascular permeability, avoiding the vascular leakiness caused by VEGF overexpression.
Doppler ultrasound demonstrated significantly enhanced muscle perfusion with HIF-1α relative to controls, which was not further augmented by co-expression of angiopoietin-1 or platelet-derived growth factor-B.

Authors

KPKatri PajusolaUniversity of Helsinki JKJaana KünnapuuUniversity of Helsinki SVSanna VuorikoskiÅbo Akademi University

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Implication

Preclinical study demonstrates stabilized HIF-1α induces functional non-leaky blood vessel growth in mouse muscle, suggesting a safer gene therapy strategy for cardiovascular ischemic disease.

Cite This Study

Pajusola et al. (2005) studied this question.

synapsesocial.com/papers/6a9bc50c3668c9fbaf954783 https://doi.org/10.1096/fj.05-3720fje

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Implication

Preclinical study demonstrates stabilized HIF-1α induces functional non-leaky blood vessel growth in mouse muscle, suggesting a safer gene therapy strategy for cardiovascular ischemic disease.

Stabilized HIF‐1α is superior to VEGF for angiogenesis in skeletal muscle via adeno‐associated virus gene transfer

Key Points

Discussion

Implication

Cite This Study

Stabilized HIF‐1α is superior to VEGF for angiogenesis in skeletal muscle via adeno‐associated virus gene transfer

Key Points

Discussion

Implication

Cite This Study

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