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September 5, 2026BMC Infectious DiseasesOpen Access

Diagnostic performance of Baermann technique, urine-ELISA, stool-PCR and Strongyloides IgG4 rapid test for diagnosis of strongyloidiasis: a study protocol

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Authors

RARufin K. AssaréSBS.C. BeckerPHPytsje T. Hoekstra

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Overview

Cross-sectional study reports diagnostic performance protocols for strongyloidiasis detection in rural populations, indicating optimal tools for large-scale disease mapping.

Key Points

  • To evaluate the diagnostic accuracy and community acceptability of the Baermann technique, urine-IgG ELISA, stool-PCR, and a Strongyloides IgG4 rapid diagnostic test for detecting Strongyloides stercoralis infection.
  • Cross-sectional survey of 1,200 randomly selected individuals across 20 rural villages in the Agboville and Dabou districts of southern Côte d'Ivoire (60 participants per village).
  • Collection and analysis of stool (duplicate Baermann, duplicate Kato-Katz, and PCR), urine (filtration, IgG-ELISA, and PCR), and finger-prick blood (IgG4 rapid test).
  • Assessment of sensitivity, specificity, and predictive values using a composite reference standard alongside qualitative evaluation of participant acceptability for each sample collection method.
  • Baseline epidemiological surveys in rural Côte d'Ivoire demonstrate Strongyloides stercoralis infection prevalence reaching up to 50%.
  • Diagnostic sensitivity, specificity, positive predictive value, and negative predictive value will be established across all four diagnostic modalities against the composite reference standard.
  • Acceptability and operational feasibility metrics will identify the most suitable diagnostic algorithms for programmatic, large-scale helminth mapping.

Cite This Study

Assaré et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd3f16b95aff0620eb2dchttps://doi.org/10.1186/s12879-026-14231-4
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Also Consider

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  1. 1Diagnostic performance of a Strongyloides IgG4 Rapid Test in detecting human Strongyloides stercoralis infection2025
  2. 2A simple parasitological technique to increase detection of Strongyloides stercoralis in Bolivian primary health care system2022
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  4. 4Assessment of Two Commercial Serological Assays for the Diagnosis and Post-Treatment Follow-Up of Strongyloidiasis in a Cohort of Patients with Chagas Disease2026
  5. 5Integration of diagnostics for Strongyloides stercoralis into the soil-transmitted helminths control programme in Rwanda2025 · 2 citations