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September 5, 2026BMC ChemistryOpen Access

Spectrum meets sustainability: green-validated novel simultaneous assays of antimicrobial fixed dose combination

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Authors

KGKarin M. GuirguisMKMiranda F. KamalEAEmad H. Al‐Dokhmaisy

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Overview

Methodological study demonstrates accurate simultaneous spectrophotometric quantification of cefuroxime axetil and ornidazole in tablets, highlighting a sustainable alternative to chromatography.

Key Points

  • To develop and validate a green, simultaneous spectrophotometric method for the direct quality-control analysis of cefuroxime axetil and ornidazole in fixed-dose combination tablets without prior separation.
  • Applied Q-absorbance isosbestic point methodology and first-derivative photometric analysis to synthetic binary mixtures across multiple concentration ratios.
  • Validated analytical parameters according to International Council for Harmonisation guidelines on laboratory-prepared tablets.
  • Assessed environmental sustainability against standard HPLC and HPTLC methods using the Analytical Eco-Scale and AGREE greenness metrics.
  • Limits of quantitation were 1.509 µg mL⁻¹ for cefuroxime axetil at the isosbestic point, 2.094 µg mL⁻¹ for ornidazole at the isosbestic point, and 2.364 µg mL⁻¹ for ornidazole using first-derivative analysis.
  • Statistical comparison showed no significant difference in assay performance between the proposed spectrophotometric methods and reported HPLC procedures.
  • Analytical Eco-Scale and AGREE evaluations demonstrated superior greenness scores for both spectrophotometric techniques over prior chromatographic approaches.

Cite This Study

Guirguis et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd4726b95aff0620ec1c2https://doi.org/10.1186/s13065-026-01790-5
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