Key result
UCP2 knockout significantly increased pro-caspase-9 expression at 28 days, while UCP2 reduced apoptotic myocytes by 70% at 28 days (P<0.05).
Why the study?
Does UCP2 knockout increase myocardial apoptosis in STZ-induced diabetic mice?
Does UCP2 knockout increase myocardial apoptosis in STZ-induced diabetic mice?
p-value: p=< 0.05
UCP2 plays a protective role against myocardial apoptosis in diabetic cardiomyopathy, as its absence significantly increases apoptotic myocytes in a diabetic mouse model.
Supports UCP2 protection against apoptosis in diabetic mouse hearts; hypothesis-generating, requires human validation before clinical consideration.
Objective: Determine the role of uncoupling protein 2 (UCP2) in the myocardial apoptosis of diabetic mellitus（DM）. Methods: DM animal models were induced by streptozotocinon (STZ) on UCP2 knock-out mice (UCP2KO) and wild-type mice (WT), which were reared for 7 and 28 days after successful modeling, respectively. The expressions of relative protein for myocardial apoptosis, pro-caspase-9, were investigated using western blot. However, the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) was used to explain apoptosis at the DNA level. Results: Image analysis showed that the expression of pro-caspase-9 protein levels increased slightly in UCP-/- + DM-7-day group comparing with DM-7-day group (P > 0.05). The expression of pro-caspase-9 protein levels increased significantly (P < 0.05）in UCP-/- + DM-28-day group comparing with DM-28-day group. TUNEL analysis indicated that UCP2 reduced the number of apoptotic myocytes in the DM-28-day group by 70% in comparison to DM-7-day group by 30% (P < 0.05). Conclusion UCP2 may be one of the most important factors that contribute to the myocardial apoptosis of DM.
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Li et al. (2011) studied Diabetes mellitus. UCP2 knock-out vs. Wild-type was evaluated on Myocardial apoptosis assessed by pro-caspase-9 protein expression and TUNEL analysis (p=< 0.05). UCP2 knockout significantly increased pro-caspase-9 expression at 28 days, while UCP2 reduced apoptotic myocytes by 70% at 28 days (P<0.05).
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