Key result
A modified chunk method using collagenase and protease without EGTA successfully isolates viable human atrial myocytes suitable for simultaneous patch-clamp and intracellular Ca2+ transient measurements.
This paper provides a detailed protocol for isolating human atrial myocytes suitable for simultaneous patch-clamp and calcium transient measurements, facilitating research into cellular mechanisms of atrial fibrillation.
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Enables simultaneous patch-clamp and Ca2+ imaging in isolated human atrial myocytes; leaves open whether this advances mechanistic AF research pending validation.
Voigt et al. (2013) studied Patients undergoing open-heart surgery. Isolation of human atrial myocytes (chunk method with collagenase and protease) was evaluated on Cell yield and viability for patch-clamp and Ca2+ transient measurements. A modified chunk method using collagenase and protease without EGTA successfully isolates viable human atrial myocytes suitable for simultaneous patch-clamp and intracellular Ca2+ transient measurements.
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